Objectives: The purpose of this study was to investigate the psychometric properties of acceptance- rejection of parents (mother and father version) and spouse questionnaire. Method: 220 married participants (120 women and 100 men) were selected using multiphase cluster sampling from 4 universities in city of Tehran and completed parental acceptance-rejection (adult PARQ), intimate partner acceptance-rejection (IARQ) and personality assessment questionnaires. Data were analyzed using confirmatory factor analysis, Cronbach’s alpha coefficient and Pearson’s correlation coefficient. Results: Content validity of questionnaires was confirmed by 3 experts. The internal consistency in all versions was more than 0.80 and test-retest reliability showed stability of scores after 3 months. The correlation of parental and spouse acceptance-rejection with subscals of psychological maladjustment confirmed criterion validity of the test. The result of confirmatory factor analysis showed sufficient fitness of 4-factor model of instrument and confirmed its construct validity. Conclusion: Overall, the finding showed good validity and reliability of parental and intimate partner acceptance-rejection instruments among Iranian students and thus can be utilized in research and clinical settings.